Microbiological Studies ..?

The purpose of building a career is not simple, easy to say, as easy as drafting on a 4A paper, as easy as preparing a laptop keyboard data and as easy as talking motivational experts to explain how to be a successful entrepreneur. When the mind is empty, the knowledge is empty, the information is empty and the pockets are empty, the purpose of stepping into any field is certainly to reflect on how difficult it is to start a career.

In this recent post, we share a little bit of brief information from my earlier research notes. While this information may not be as important as it has been, it is to me a very difficult piece of information that is rarely taken care of by the gaharu. There is no mistake if we are constantly hungry for knowledge, no matter whether the information is accurate or not, it can be evaluated through our research.

The formation of algae has substances involved in starch and sugar metabolism based on the invention of the electron microscope, it is found that starch granules exist in healthy wood, but when the wood is injured the oleoresin of the gaharu accumulates while degrading the starch content. In natural forests 7% are infected with fungus (Crous 1996) through pathology, wind, insect attack or microorganisms between fungi: - Ascomycetous, Phaeoacremoniu and Parasitica.

The ingredients of the ingredients are in the following tree: - Sesquiterpenes (Q-guaiene, Q-humulane and 5-guaieane), Derivatives of phenylethyi chromone, Oleoresin, Terpenoid and Syloluose.

However, little is known about the fungus associated with the development of the symptoms of the disease and the formation of the newborn, from the study to produce and identify the Pathogen in Aquilaria spp dieback disease, Lasiodiplodie theodiplomael, in a pathogenicity test confirming that L. theobromae is a natural pathogen Aquilaria spp and due to the production of gaharu.

In this study to test the quality of the algae derived from Aquilaria spp due to fungal - (F) chemical composition and relative amounts of essential oils measured by gas chromatography- mass spectrometry (GC-MS), using wild-type (W) and principal- Healthy stems (H) as positive and negative controls, anti-fungal activities of essential oils derived from the specified algae.

Micrological studies 1.

A. 4-year-old Aquilaria spp was injected with a 0.4cm vertical drill 1cm deep in the stem, a seven-day fungal species (F) inserted (L.Theobromae) breeding on "agar" potato dextrose (PDA) around the wound. closed drill holes (bamboo stick). The use of PDA has been used as a negative control.

B. After 6 months of injected fungus (F) was harvested for oil isolation, a 20cm long stem was collected and a hole in the middle of each treated stem, and the skin was extracted and submerged in liquid nitrogen stored at −80 C for storage. GC-MS analysis, obtained separation oil after weighing (100 g).

Composition and anti-microbial activity of essential oils derived from A. ginsensis (Lour) was driven by the algae biological agent, Theobromae Lasilodiplodia (F) as compared to wild-type (W) between healthy and non-microbial (H) plants as positive and negative controls. After testing using gas chromatography-mass spectrometry (GC-MS) the essential oil (F) showed a similar W composition with sesquiterpenes and aromatic constituents.

The essential oils of H, found in alkanes, the essential oils of F and W are more potent inhibitors than L.theobromae, Fusarium oxysporom, and candida albicans than essential oil H. The findings show for the first time that essential oils derived from algae are from A. sinensis caused by L. theobromea has a high affinity with wild algae oil.

Both in chemical composition and anti-microbial activity, in addition to the strategy of fungus, fungus is potentially used in Aquilaria spp and for the production of essential oils.

In two phytopathogenic (F) experiments (phytopathogenic (lasiodiplodia thyeabromae and fusarium oxysporum)) and one clinical fungus, (candida albicans ATCC10231) was used as a test of the organism L.theobromae was a pathogen sinensis dieback was used as a pheasant species. 18F, oxysporum is a phytopathogenic fungus isolated from ginger samples has been identified based on morphological microscopic analysis. the mycelia and the conidial spores, with the use of key diagnostics of 20 C, albicans, agents that cause oral opportunism and sexually transmitted infections in humans.

Anti-fungal activity of essential oils was evaluated by method and “agar” dispersion, with a modification of 21.22C, albicans were grown in a 28 ”C medium extruded potato (PD) liquid and diluted spore suspension (10 5 mL-1 spores) was obtained. prepared for cleavage F, oxysporium and L. theobromae were maintained on PDA at 25 ”C. The spores were prepared from 7-day incubation, the tested fungus suspension was prepared (10 Ml-1 spores) and added (100 mL) to the “agar” plate and distributed evenly over the surface.

Small wells were cut into “agar” plates using sterile cork-diggers (6 mm) and 50 ml of oil solution, at a concentration of f50mgmL-1 dissolve sufroxide (OMSO) was added to the well.

Negative controls were provided using OMSO only. Fluconazole (200 g mL-1) was used as a standard as it is clinically used as an anti-mycotic drug. The plates were incubated for 48 hours at 3% ”C, albicans and 25” L.theobromae and F.oxysporum Diameter of the surrounding zone was recorded in 4 different directions.

Minimum inhibitory concentration (MIC) concentration of fungus (NFC). The value of essential oils to L. theabromae, F. oxysporum and C.albicans was determined based on the 23,24 micro-dilution (EM) dilution method.

Studies have shown that fungal species, such as Aspergilus sp. Botrydiplodia sp. (Lasiodiplodia sp.) Diplodia sp. Fusarium bulbiferum. F. laterium. F. oxysporum. Penicillium sp. Pythium sp. Trichoderma sp. It is common to infect Aquilaria species through pathology rather than pathology. The samples studied were taken from healthy plants to produce up to 49% of hexadecanoic acid and sesquiterpernes contributing 8.4%.

The causal agent of causation can be divided into physical, chemical and biological agents, out of the three agents, whose biological role of acne inoculation, using fungus, is encouraged as it leads to the development of the algae.

The most efficacious of several applications in the production of algae has been tested by mimicking natural processes, although there are reports of algae quality due to fungal induction. To our knowledge, the first report of fungal induction (inoculation) produced 34% sesquiterpenes and 4% aromatic and formative compounds in Aquilaria, with the review that their plant defense mechanism was due to the production of guava.

Comparing scientific knowledge and traditional knowledge of indigenous peoples and other forest products collectors, from the South Asian and southeastern Asian countries, its management is so limited that it fails to supply large numbers of gaharu, through cultivation, it is found that indigenous peoples recognize the complex ecology of resin-forming complexes two factors or maybe three, a living-organisms, one or more fungal infections and possibly insects as intermediates.

The development of a comprehensive production system for these resources requires a clear understanding of how the various natural elements function separately and synergistically. Traditional knowledge can help fill in the gaps in information base and identify areas as additional information for future research studies, research reports are, after all, considered as gaps in opinion.

But. can support as basic knowledge, will play a major role in integrating ethnobiological research, especially between ethnobiological and forestry in developing a sound management system for traditional resources and natural habitats.

In 1977, Philiophora parasitica was identified by researchers, reports saying that the tracheid wall cavity of a tree organism Aquilaria spp. shows that the most common fungus found during isolation includes Pencillium ciitrinum, Aspergillus tamani and Aspergillus spp including Fusarium solani, Botryadplodie theobromea and other unknown fungi, but there are some reports that Phialophore parasitica is often associated with certain parts of Aquilaria spp . to produce a quality algae.

The formation of a giraffe is rarely found in trees aged 25 and under (theoretically), if found to be probably due to tree injuries, for example due to storms and fractures, it is attacked by fungal infections, if the tree is in good condition, aquilaria spp at this age, scented oleoresin will be found but as traditional techniques cut, not on the stem, the oleoresin begins to oversee the injured parts and will be exposed to fungal infections.

The isolated Cytosphaera mangiferae fungus found on dead standing trees, one of the fungi invading dead trees in a dead state, is known to identify four other fungal species associated with aquilaria spp. (Aspergillus sp. Fusarium sp. Penicililium sp and Epicoccum sp).

In 2000 researchers identified two fungal cultures found in the diseased timber of Fusarium oxysporum schlect and chaetum globosum kunze, and had successfully inoculated the healthy tree with the aim of growing the colony.

From 1991 Indian scientists have successfully infected the infection, initiated this artificial infection program under cultivated aquilaria; , however, the increase in the production of new inoculation grains could help boost global demand.

In order to obtain the formation of wild or natural reeds, the tree must be at least a year old or thousands of years old and must possess a large number of trees. It relies on 100% of insect attacks (ants) to make nests in this tree, broken by wind or trauma and microorganisms attack from the ends of the branches to determine the natural ability of the wildebeest.

We can imagine the actual amount of production of natural eagles and how the current global trade, 99% of which is from inoculation eagles, seems to be non-natural from the formation of nature.

There is only a difference in quality between natural and inoculated algae. Trandsional methods such as scarring, burning, drilling and traumatic events, but popular ones, although considered to be the latest, are used in the injection of inoculation fluid (avocado or vaccine or liquid vaccine) or are defined as human steroids. TQ.